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There are some improvements bug fixes we can make to the single cell visualization.
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First of all, thank you very much for writing this nice piece of tool. We have been running it for a while and there is this rare situation where `readfish` freezes. That is , no more log messages lik…
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Based on the guide of generic-amplicon preset, the reads are constructed as follows.
The read outputs that I am trying to process through MiXCR, however, looks like this. R2 has the UMI sequence…
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Hi, I'm running canu 2.2 on a partitioned slurm grid. I have gotten to the consensus step but I'm consistently getting an issue at (I believe) the consensus step. The script calls 'utgcns' which start…
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canu command run:
```
canu -d assembly/R5_Diff -p R5_Diff -pacbio-hifi trimmed_reads/R5_Diff.fq useGrid=false genomeSize=3000000 maxInputCoverage=20000
```
output of canu -version:
```
can…
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Hello, I have HiFi metagenomic reads from a low complexity sample. I first tried running canu but very few reads were retained, I ran again following suggestions from this [issue](https://github.com…
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https://mp.weixin.qq.com/s/q5nGX8mNRMRbj6UvusQ_eg
ixxmu updated
7 months ago
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## **Is your feature request related to a problem? Please describe.**
One important problem to be solved is how to split a sequence into N fragments in a way that it could be mounted again later. A g…
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Dear,
We are testing UMI-adapters (5M2S on both sides) in combination with our current workflow (2*75bp sequencing).
For bio-informatic analysis afterwards, we relied on many fgbio tools.
Conc…
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Hi,
When I run mixcr, I get this warning:
WARNING: No tags with type Molecule in data (-uniqueTagCount Molecule)
Can this be caused by incorrectly running mixcr?
Thanks and good day.