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Dear Aaron,
I'm trying to index T2T-CHM13v2.0 (https://s3-us-west-2.amazonaws.com/human-pangenomics/T2T/CHM13/assemblies/analysis_set/chm13v2.0.fa.gz) with FlashFry:
java -Xmx10g -jar FlashFry…
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I've been talking with lots of people about what would be most useful to add in version 2 of SPICE. I've gotten lots of great suggestions. Here are some ideas in no particular order.
- **Solvated…
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**Describe the bug**
pyrodigal does not work:
```
[ERROR: Pyrodigal could not be executed! Please make sure Pyrodigal is installed and executable or skip requiring workflow steps via via '--skip-c…
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Hi,
Really useful package, amazing work, always glad to see others (also) filling research gaps with open-acess code! 💪🏼
As some parts of the documentations were rather ambiguous (I am not that …
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Hi experts,
I recently retrospectively find out my amplicon has a lot of primer dimers [10-20%]. But I never had any problem with crispresso (awesome), my WT sample is always
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See original description and sample URLs in [SF #00022666](https://dryad.lightning.force.com/lightning/r/Case/5003h00000bUQe2AAG/view)
Some PPR links are not working correctly. They bring up the page…
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Hi, I am learning the Mixscape function in the Seurat.
Could anyone let me know how to import single-cell CRISPR screening data into Seurat with meta.data information?
In the Mixscape vignette, the …
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Dear sir,
When I tried to use :
"CRISPRessoWGS -b 1K/PCR-KL20211111-ALAS1-1_S52_L002.sorted.bam.bam -f guide.txt -r ALAS1_ngs1k.fasta"
It shows massage like
~~~CRISPRessoWGS~~~
…
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Hi, I am using NFCORE MAG for the first time and unable to understand the error I ran through with prokka. I have attached the error log below. I appreciate your help.
Thanks
Anu
The full error m…
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When running the example from the Demo folder with iPython, I keep getting this error trying to create the plots interactively
```
In [1]: run screen_analysis.py
In [2]: data = loadData("Demo/Ste…