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Apparently, dorado v0.4 and newer versions have added a chimeric read splitting feature. However, it does not reach 100% sensitivity. I was wondering how is exactly the read splitting working. Assumin…
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Hi all,
we have done basecalling with Dorado with the following command:
dorado basecaller rna004_130bps_sup@v3.0.1 --modified-bases m6A_DRACH > output bam.bam
Do I have to search for the DRA…
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Hi There,
I ran Pychopper after Dorado SUP basecaller v0.7.2 in a Linux server.
I used a command similar to:
```
pychopper -r ${SAMPLEID}_report.pdf \
-k LSK114 \
-S ${SAMPLEID}_stats.tsv \…
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Hi there,
Thank you for developing this handy tool; I've been using it for a long time. Recently, I wanted to perform a more challenging test on my tool by adjusting the read length of the simulate…
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Currently I am researching ONT possibilities with cutadapt, and it seems that the most basic functionality can be achieved. Unfortunately after the adapters have been adequately cut, sequali still fin…
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Hello, I used to run snakemake on the UGE cluster using --drmaa " V -cwd -l gpu=1 -pe mt {threads}"', in the new versions this option is deprecated, so I installed a [uge profile](https://github.com/m…
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I merged the bam files from the modified 5mc and 5hmC from Dorado with samtools merge, I index and sorted it with samtools as well.
when I try to run methylartist segmeth with this bam file as my -b …
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I'm trying to extract methylation data from bacterial genomes using Dorado 0.5.0 and modkit 2.4 (using the bioconda hosted package).
I've aligned my reads using Dorado align and sorted/indexed my …
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https://d.ymdd.tech/1.0.0/
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http://localhost:4000/