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Hi,
Firstly, thanks for the great tool, it's very helpful in choosing the cutoff parameters for DADA2.
I have a dataset which was very deeply sequenced and as far as I can tell the reads are no…
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Hi, Professor Wang@wtwt5237
Sorry to bother you. thank you for doing such an excellent job! I am trying to use the "QBRC-Somatic-Pipeline" in my deep exome sequencing data.I see that the software …
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Hello Sir/Ma'am,
1] Trying to apply oncocnv for exome data i.e. without reference to amplicon id. What should be modification required in bed file for applying it on Exome data.
Standard bed …
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Hi all,
We are sequencing minION flowcells using the ARTIC amplicons and beginning to test out the ARTIC workflow as described on this page:
[ARTIC SOP 2019](https://artic.network/ncov-2019/ncov…
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Bigger milestone to start as separate repo but version of pipeline to support minion and pacbio with test data for both
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We have examples where there is a choice of two primers for the end of a given amplicon. Only one primer of the two primers has support from the reads.
Observed behaviour: Both primers are kept, in…
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'Omics terms can be difficult to learn. Having them described in a single page may help new comers to overcome this barrier quickly and transfer knowhow that may be a pain to transfer through more for…
meren updated
2 years ago
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Hi @Meghana9854 @roseedwin , I just came across your amazing paper, and I wanted to implement the approach you proposed for my soil shotgun data. Looking upon your methodology, it seem you relied on a…
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**Describe the bug**
I'm working with SARS-CoV-2. I only want to incorporate indels into a variant call file with `ivar variants` and consensus genome generated with `ivar consensus` when a certain f…
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Hello,
I am trying to run the ARTIC pipeline on the Singularity profile with the new ARTIC V4 primer scheme set (https://github.com/artic-network/primer-schemes).
I am running the Nextflow pipeli…