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1. multiplex
2. quality check
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In the current [Support Matrix](https://github.com/disq-bio/disq#support-matrix) there is no mention to the status (currently none) to FASTQ-files. Although HadoopBAM has it but it is not widely used,…
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### Description of feature
[cellranger_count.py](https://github.com/nf-core/scrnaseq/blob/master/modules/nf-core/cellranger/count/templates/cellranger_count.py) currently just uses `subprocess.run` f…
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**Goal**
Development of a stand-alone fastq validation tool.
A flexible tool that can be applied to different single-cell technology types to check for the expected cell barcode.
It should also r…
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Where I can dowload UMI.fastq.gz file as you say in your pepline?
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fastq-sample segfaults while subsampling a couple of fastq files:
fastq-sample -n 900000 -o subsample first.fastq second.fastq
...
open("subsample.2.fastq", O_WRONLY|O_CREAT|O_EXCL, 0600) = 6
fcntl…
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I am using FASTQ reads as input for Acacia. I was expecting that the reads in the output would also be in FASTQ, but they were in FASTA format. How hard would it be to implement FASTQ output?
Cheers,
…
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I am encountering an issue with basecalling a POD5 skip file using Dorado. I have two POD5 skip files generated during a GridIon sequencing run with a P2 solo attached. These files were generated by p…
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Hello!
I'm testing vRhyme and got stuck after 'Performing machine learning classification' step
Running on a slurm HPC system
Fresh mamba install
Inputs :
a) Single-end Next-seq reads;
b) vir…