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Thanks for the good material. I have a question about the alignment using bowtie2, here according to [`mutation_sequencing_analysis_script.sh`](https://github.com/aldob/iMUT-seq/blob/4476db76f2058d0af…
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Hi,
I tried RNA sequencing fastq data alignment by STAR 2.7.5a.
The below is command.
STAR --runThreadN 12 --twopassMode Basic --genomeDir /home/users/sijaewoo/common_files/hg38/STAR --readFile…
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Hi Developers,
How to set the Varsim parameters to simulate a single-end file of shallow whole-genome sequencing (0.5x) given mean fragment size of 167 bp?
Best
Pitithat
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Dear Unicycler team,
I chose Unicycler to assemble _de novo_ bacterial genomes with ONT sequences, because I tried with several ones and Unicycler gived me better results.
However I start to obser…
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Hi,
Thank you for the great tool. I want to use an ONT long-read to assemble the mtDNA of _Spirodela polyrhiza_. I have the following error. How can I change the option of PMAT to pass the error m…
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While running the wf-human-variation pipeline, ont-spectre produced an error implying that either the vcf file and reference files were empty or that the script did not load them correctly. I have at…
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Hi!
I am currently working on the IDseq metagenomics platform to identify mapped reads to SARS-CoV-2. Basically my project consists on the sequencing of all reads from a clinical samples with COVID-…
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Hello,
I would like to use your software on sequencing data aligned to the newest R. norvegicus genome (RGSC 6.0/rn6). Could you please add it to your annotation database?
Cheers,
alussier17
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Related to https://github.com/nextstrain/private/issues/102
It seems like a common pattern for sequencing efforts to focus on specific genes instead of the full genome. It would be helpful for inge…
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Hello,
Can the same pipeline be used for cDNA sequencing as well? I have the fastq reads and the reference genome and transcriptome fasta sequences.
Thank you.