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# Background
New library construction technologies, especially highly multiplexed assays like single-cell RNA-seq, employ a large variety of library molecule configurations. These configurations inse…
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Hi,
I'm trying to do RNA velocity analysis in python.
I have already installed scvelo and other relevant packages in conda (python version 3.9).
I extracted count matrix using following code in R:…
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When I attempt to run cellrank in a conda enviornment that also contains velocyto (so that I can generate my count matrices and then proceed) I am running into an issue where cellrank claims it has no…
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We have multiple datasets that we need to prepare:
# multimodal single-cell CRISPR screens:
- [ ] https://nature.com/articles/s41588-021-00779-1
- [ ] https://www.nature.com/articles/s4…
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An increasing number of single cell transcriptomics are identifying cell states that are transcriptionally unique. CL editors have decided on the call of November 2023 that these cell states can be ad…
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https://mp.weixin.qq.com/s/7Svp-2iuzQyqONpEp4bqOg
ixxmu updated
3 months ago
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https://mp.weixin.qq.com/s/7Svp-2iuzQyqONpEp4bqOg
ixxmu updated
3 months ago
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Hi @WeilerP
When I am trying to run scv.pp.filter_and_normalize(NDN)
I get the error "AttributeError: module 'scvelo' has no attribute 'pp'"
my annData objetc(NDN) looks like below.
AnnDa…
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I'm opening this issue here because I believe it's more of a pufferfish-related matter, but will use the issue template from your salmon repository.
**Is the bug primarily related to salmon (bulk m…
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Hello Delaney:
Is it possible to run that kind of analysis on BAM files instead of FASTQ?
[Here](https://www.reddit.com/r/bioinformatics/comments/ih4bdk/kallisto_analysis_of_bam_files/?rdt=54029…