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Hello,
I am building the reference for IRFinder using a SGE cluster, submitting with the following params
```
#$ -l tmem=50G
#$ -l h_vmem=50G
#$ -l h_rt=92:00:00
#$ -j y
#$ -N irbuild
…
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Hi @marcus1487, I was using remora API on real direct RNA-seq data, but ran into errors from this command:
```python
io_read = Read.from_pod5_and_alignment(
pod5_read_record=pod5_reads[ba…
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Is it possible to implement a utility function within the Salmon binary that converts the transcript mappings to genome coordinates of an input GTF file?
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- [ ] ned1 as per PMID:26774782
The second
annotated 150bp-long intron is retained, resulting in insertion of additional 50
amino acids in a region between the N-lipin and HAD-like domains
old
…
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Hello,
I am trying to map a list of fastq files to reference mus musculus for my research. My system has 32GB ram and i7 processor. But when i start running i get the following error. I don't have an…
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There are substantial spliced alignment.
Should we prevent spliced alignment in non-genomic alignment?
Does hisat2-3n has that type of options?
hwlim updated
5 months ago
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Minimap2 allows you to use -ax splice mode for spliced alignment and a way to add junction bed notations. As far as I can tell this isn't included in the current dorado release. Are there plans to ena…
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Hey, many thanks for this awesome tool !!!
I try to fine tune for _Arabidopsis thaliana_ with more RNA seq data.
Below are the commands I used, but I got the error when I ran filter-to-most-certa…
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_From @olgabot on July 6, 2014 22:55_
Hi @jbenet and @maxogden! Thank you so much for the time you took to meet with @mlovci and me this weekend. Here's an overview of our current data management sit…
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The Sashimi plot is a popular feature in IGV, lets do it for igv-web.