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Hello
I am using GECKO library and I was wondering how I can get a sgRNA library file for the input.
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I am wondering if it is possible to use an index built from just fasta files containing the gRNAs used in single cell pooled CRISPR screen. I can quantify gene counts using STARsolo. But if I try to u…
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Hello,
I am using Jacks to analyse a CRISPR screen where i want to select positively enriched genes compared to a control.
Everything works very well in terms of beta scores, with a positive cont…
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The new BAGEL v2 not only adds cross-validation, but uses it by default instead of the bootstrapping method. I assume you compared the two approaches. Is that information available somewhere? Is there…
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When I try to download the input file for hg38, it says "you do not have permission to access this server"
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Hi GEMINI team,
I use your tool to analyse a combinatorial CRISPR screen. I run the dataset from the tutorial (http://www.bioconductor.org/packages/devel/bioc/vignettes/gemini/inst/doc/gemini-quick…
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Hi,
Thank you for this very useful package. As many of the CCLE lines have now been screened in Achilles experiments, I'm wondering whether any thought has been given to harmonizing and serving th…
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Hello everyone,
I am here in search of help to know the tool mageck better a s I am facing problem in allignling my reads on library file. ZerosgRNA is having 0 count. Can you help me in this??
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Hello cancerdatasci team,
I read the publication for CERES by Meyers et al. on Nat Genet, and I am excited to try CERES to correct for copy number effect in our CRISPR KO screen (which didn't use G…
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Are there any plans to include more recent releases? I know they changed the format of their data releases pretty significantly, but there's also been a significant amount of new data added for some d…