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Should be possible to add several guide RNAs and a single repair DNA (if any)
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For the guide-pooled screen,article focused on the 8,448 cells with 3 or more guides. When I was follow ,there are some issue. effect size is NaN or 0.
[/nis_home/FR-Perturb/bin/python /locald/FR-…
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The search option gives me an ID for a given gRNA sequence. Is there also a way to retreive the CRISPR sequence and location computed in the get_all_crisprs step?
Also, is there a way to retreive the…
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I'd like to be able to design CRISPR guide RNAs with Poly. Below is a draft spec for a new CRISPR package:
## Spec
Our guide RNA design package should be lightweight and easily extendable and at…
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It would be nice if crisprVerse can support DjCas13d!
> Wei, J., Lotfy, P., Faizi, K., Baungaard, S., Gibson, E., Wang, E., Slabodkin, H., Kinnaman, E., Chandrasekaran, S., Kitano, H., Durrant, M. …
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Dear Ben,
Did you try the Implement of maximum-likelihood estimation for true-indels in CRISPR in Dr. Feng Zhang's method in Nature Bio-technique paper (DNA targeting specificity of RNA-guided Cas9…
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The full list of targets is available here
https://github.com/jump-cellpainting/jump-cellpainting/blob/master/0.design-pilots/output/drug_target_samples.csv
These 7 compounds are not listed in tha…
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Hi There,
I am using lofreq to the identification of CRISPR off-targets however when the lowfreq is throwing a warning in the end.
WARNING [2022-03-11 23:33:27,130]: uniq stderr: WARNING(lofreq_u…
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The new manuscript on project score is very close to be published. The dataset is available, and we can update our parsers to ingest.
- Parsers for generating project-score evidence is [here](http…
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As we consider what the must-haves and nice-to-haves are for a CRISPR-Cas kit: should we be providing an expression device/plasmid for gRNA paired with a particular Cas? This may all be moot if we wan…