-
Should be possible to add several guide RNAs and a single repair DNA (if any)
-
Add package with parts (CDSs, UTRS, SPs, etc.) for plants
-
Hi, thank you so much for developing this fantastic MAGeCK package, its really helpful in analysing CRISPR-screen data. However, I am facing issues when I try to analyse the data using the paired end …
-
We use STAR Solo to map results of CRISPR screens, specifically at one of the steps we map guide RNA reads that we have introduced and assign them to cells . For that we generate artificial reference,…
-
I was having trouble running the script: `CRISPR/download_data.sh`. In debugging, it turns out that the tables are no longer hosted at the URI in the script.
See: [https://images.nature.com/origin…
-
This SEP proposes a set of terminology and practices for representing genetic parts and functional devices at various stages of design, synthesis, and assembly. These practices are intended to represe…
-
Hi,
I am trying to use crisprVerse for paired gRNA design. But when I ran the `findSpacerPairs `function, it returned an error.
```R
> data(SpCas9, package = "crisprBase")
> bsgenome pairs upstr…
-
Hello!
I was screening for off-targets using gRNA sequences desgined to target a transgene that normally does not occur in my organism.
In the summary.xls, I realized that when a gRNA is not found…
-
Hi, really appreciate for the tools provided by crisprVerse team. I tried to score different sgRNA libraries using [Validating Existing gRNA Libraries tutorial](https://github.com/crisprVerse/Tutorial…
-
**Describe the bug**
Status report
Indexing genome(s): Not available
Searching spacer: Not available
Post processing: Not available
Merge targets: Not available
Annotating and generating images:…