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Hi,
I have Pacbio FLNC reads in fastq format. What options should be specified while running the tool. I was thinking
`--data_type pacbio --fl_data`. Is this correct?
Thanks
Abhijit
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Hello,
I am currently trying to run finder on three whole genome samples:
1. Sequenced with Illumina HiSeq x ten
2. Sequenced with Illumina Novaseq 6000
3. Sequenced with PacBio SMRT
Samples…
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We're just getting in our first standard 16S data from the PacBio Revio which is using the [Kinnex kit](https://www.pacb.com/technology/kinnex/), and will soon be getting 16S-ITS-23S data. I know the…
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Hi,
I am trying to use genomescope to estimate heterozygosity and genome size of a fairly large plant genome, ~ 3.65Gbp. I am using the histogram of canu-corrected reads. Here is what I am getting:…
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Dear Developers, hello!
There are some projects using PacBio single-molecule long-read sequencing to analyze full-length transcriptome, but the raw data is in a bam type file and has to be changed in…
wu116 updated
1 month ago
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My problem is that I am having difficulty obtaining a list of variants using bcftools mpileup and bcftools call with PacBio data. I do not have this problem with data of Illumina paired-end sequencing…
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It looks like this set of analysis tools is set up for analyzing paired-end sequencing data. Is it also possible to analyze long read data (ONT, PacBio), which aren't in paired-end format?
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Hi
I am trying to use the metamos software to analyse some pacbio data. When I try to run the pipeline I get this message:
Starting Task = preprocess.PREPROCESS
**\* metAMOS: Error: cannot correc…
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Hello,
I want to download the PacBio bam.
for example:
wget ftp://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/HG002_NA24385_son/PacBio_MtSinai_NIST/CSHL_bwamem_bam_GRCh37/B…
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Hi @mir-cat,
I'm using graphasing and have generated a rukki paths file for my sample.
```
name path assignment
haplotype2_from_utig4-2136 utig4-0[N5000N:ambig_path]