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### 路由地址
```routes
/zhihu/people/activities/:id
```
### 完整路由地址
```fullroutes
https://rsshub.app/zhihu/people/activities/diygod
```
### 相关文档
https://docs.rsshub.app/social-media.html#zhi-hu-yong…
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Dear Author,
We use ONT amplicon data to detect target insertion by CRISPR. We expect at least 2 alleles: WT (no insert) and with insert:
WT: upstream flank Cas9 cutsite (1kb) + downstream f…
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**Submitting author:** @nikola-sur (Nikola Surjanovic)
**Repository:** https://github.com/Julia-Tempering/Pigeons-Paper
**Version:**
**Editor:** @pitsianis
**Reviewers:** @nsailor, @georgebisbas
**M…
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Hallo, I want to find the functional consequences for the variants in vcf file. I have been using bcftools csq, however the problem is, that I want to use ucsc and have been using gff3 from Ensemble. …
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https://m.cnbeta.com/wap/view/1330161.htm
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Hi, Seurat team
I'm trying to analyze a Perturb-seq dataset following the mixscape vignette.
I encountered two issues:
1. After `RunMixscape()`, R console show several messages "WARNING! NOT CO…
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New Term: Negative regulation of CRISPR-cas system
Definition: Negative regulation of an adaptive immune response of bacteria that serves to clear host cells of foreign DNA and RNA. It has three dist…
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https://mp.weixin.qq.com/s/Bx0LKZCX3cPXzlBiFVIZlQ
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Hello @aaronmck I have been using FlashFry a lot and it's a very useful tool. I am looking to count sgRNAs on sequencing reads (10+ million) that I use as reference sequences for indexing. When runnin…
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Hello,
I am using PEPPER-Margin-DeepVariant pipeline in order to phase reads from CRISPR/Cas9-mediated-targeted enrichment nanopore human data with the following code (find image attached).
![Scre…