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I run miRge3.0 like this，with Qiage UMI:
`miRge3.0 -s SRR13077007.fastq -db miRBase -lib miRge3_Lib -on human -a AACTGTAGGCACCATCAAT --qiagenumi -umi 0,12 -o output_dir -cpu 10 -udd`
Is the read…
hy101 updated
9 months ago
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Which versions of miRBase and miRGeneDB where used to create the provided libraries (https://sourceforge.net/projects/mirge3/files/miRge3_Lib)?
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Hi Dr Axtell,
I predict de novo miRNAs with SS 4.0.2 and the analysis didn't work for one of my bam files (otherwise SS works perfectly for the others). On the other hand, I have previously predict…
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Hi, I am using the ShortStack version 4.0.2 and by running the command: ShortStack --genomefile genome.fasta --readfile B1_clean.fa --dn_mirna --known_miRNAs known_miRNAs.fasta
It seems something is …
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https://mp.weixin.qq.com/s/f-NdvCMEIS35eckTjA2c9w
ixxmu updated
4 weeks ago
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Here's my command:
```bash
ShortStack \
--genomefile "${genome_fasta}" \
--readfile ${trimmed_fastqs_list} \
--known_miRNAs ${deep_dive_data_dir}/${mirbase_mature_fasta_version} \
--dn_mirna \…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/jacopo-ronchi/MIRit
Confirm the following by edit…
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Hello,
when analyzing cluster properties of my sRNA data, I have encoutered this error:
```
_csv.Error: field larger than field limit (131072)
```
I have found this solution to work, when ad…
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Hi dear,
I am working on miRNA quantification using the ```quantifier.pl```. My requirement is to perform quantification using mature and precursor sequences (in fasta format) obtained from MirGene…
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Results have been [aggregated](https://nbviewer.jupyter.org/github/bebatut/galaxy-training-m…