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Hi,
I am trying to run prokka from the docker image, and keep encountering the error that my input '* is not a readable non-empty FASTA file'. The files I have tested are non empty, readable, and i…
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Dear author:
I tried to build the environment with dependencies in conda and found that there will a conflict of python version 2 and 3.
Now, after many attempts, I was able to build a docker wi…
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```
Dear Torsten Seemann,
I was wondering if it's possible to get some kind of score from Prokka.
I looked through the code and documentation and while it's possible
to define the evalue t…
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Hi!
Lately, I experienced fragmented gene annotations in terms of two (or more) ORFs that are in close proximity and are annotated with homology to the same gene. In such cases, Prokka ads an `_n` …
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There is a small issue where one of the biopython functions has a character length limit on sequence IDs, a more informative error message might be useful. A fasta ID
```
>SEQID_TOO_LONG_BIOPY_HAS_C…
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Hi Torsten,
I noticed Barrnap doesn't annotate well rRNA. My genomes have an intergenic spacer region of 150 bp in 23S rRNA and PROKKA annotates different CDS in that region. For that reason, no 23…
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Dear tseemann,
I want to only annotate plasmid sequences from an assembly file (contig.fa). Can you please guide how can i do this?
Thanks
J.A
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Hi,
I tried both manual installation and conda installation for metawrap 1.2.1, both came across this issue when following the annotate module:
```
/user/tools/Miniconda3/envs/metawrap-1.2.1/lib/…
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Hi,
I was following this tutorial and I met problems when running roary on .gff produced with Prokka.
Here's the message:
Warning: unable to close filehandle $bed_fh properly: Disk quota excee…
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Hi guys,
Thanks for the good work,
Using a cluster computer, I was downloading your databases the full version and it stopped at step 14
2021-10-30 20:29:18,929 [INFO]: Step 14
2021-10-30 20:…