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http://jvi.asm.org/content/81/4/1619
sequencing classified as "sanger". Note: all sequences were supposedly obtained with an HLA-B*57:01 tetramer, but some of the individuals are actually HLA-B*57:03…
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Hi,
I am running the first part of the analysis on the example bam file (downloaded from drive) and I get different results. This is the command line:
```
qgetcounts -i M62_NA13019.bam -a CN…
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We've got manganese chloride (MnCl2) borrowed from the Dedon lab. Also have MnSO4 ordered in.
1. Make up 10 mM stock solution of MnCl2.
2. Set up PCRs (say, PB2 Part 2, which is well within what we ca…
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Hi,
When I was using mergeSAM.py to merge the two mapped reads file and get the pairing statistics, the results showed my pairs are 80% multiple pairs which is not reasonable. And I checked the scrip…
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if possible, please verify method.identification column: filled in "antigen-loaded-targets,cultured-T-cells",
based on below methods section:
"Antigenic stimulation was performed on thawed PBMC. Indi…
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I wanted to create some primers to insert one DNA segment (GFP) into a vector (pUC19). I assume I am supposed to use `integration_primers` here, not `assembly_primers`?
`p1,p2 = pydna.integration_pri…
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Can this be a feature type to describe the staggered
dsDNA ? the range of the feature could then describe
the number of bases by which there is a overhang
Reported by: jyotikhadake
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Can this be a feature type to describe the staggered
dsDNA ? the range of the feature could then describe
the number of bases by which there is a overhang
Reported by: jyotikhadake
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def: "In uracil interference assays, the DNA will be amplified by PCR in the presence of a mixture of TTP and dUTP to randomly replace thymine by deoxyuracil residues before the binding assay. If T nu…
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Hi,
I'm reading a paper where they find human myosin 6
coimmunoprecipitates with RNA pol II at the promoters
and/or intragenic regions of active genes (from
crosslinking chormatin followed by QT-PCR)…