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I'm processing our first Element AVITI 16S amplicon sequencing data, using dada2 in QIIME2. I'm wondering how to do this optimally, as it appears that it behaves a bit differently from MiSeq data in d…
mniku updated
2 months ago
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External workflows, e.g. the once from the training material, should be updated regularly. Or even better should be a linked against the training material. Maybe we can pull them down before running t…
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Dear Developer,
Firstly, thank you for this fantastic R package. I am working with low microbial biomass samples from the nasopharynx. We are using whole metagenomic sequencing data to characterize…
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### Operating System
Other Linux (please specify below)
### Other Linux
Ubuntu 20.04
### Workflow Version
wf-amplicon v1.1.1
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow ex…
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I have another question about the Excel workbag for version V3, but I found that the link is invalid. Is there still this Excel workbag,thank.
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cutadapt 4.4
python 3.10
pip 22.0.2
I am using Cutadapt to demultiplex my amplicon sequencing data, and I am reporting the number of amplicons detected under adapters_read1 in the JSON report, af…
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"Hey, thank you very much for this cool tool. I'm working on amplicon sequencing derived from public data with taxa at the genus level. According to the instructions, the only mandatory input is the a…
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I'm running ControlFREEC with targed NGS data and no control data and in order to normalize I attempted to run the specified on the website I generated a GC-content profile with gccount.
But even wi…
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## Checklist before submitting the issue:
- [ ] The issue is strongly related to the MiXCR software
- [ ] The issue can be reproduced with [the most recent version](https://github.com/milaborato…
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Greetings! I have a Fasta file containing a few dozen amplicon sequences, and I would like to use InSilicoSeq to simulate targeted sequencing of these amplicons. I had originally used `--mode kde --mo…