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When trying to figure out how to document metabarcoding data, I came across some things that were not super clear to me.
Any insight in this question is much appreciated, and perhaps the terms could …
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Hello!
I am using cutadapt to demultiplex my reads (v2.10 with Python 3.7.6).
Basically, I have a pair of fastq files (e.g. input_R1_001.fastq and input_R2_001.fastq) which contain sequences fro…
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I have a fastq file with 1.7 million reads of 75 bps, and 28 different barcodes. My command:
``fastq-multx -l barcodes.txt all_samples.fastq -e -o %.fq -x"
Part of my barcode file: barcodes.txt:
…
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Hi,
I'm trying to use dada2 to analyse PacBio 16S full amplicon data. I only have four samples which I filtered with these settings:
```
> track2 print(track2)
re…
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Dear,
I am leaving the Nanopore Day in Bordeaux and there Stephen Rudd presented the release of this promising tools.
What should be the size of the training set? Number of reads of the same ref…
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Hi,
while looking for tool to analyzie our CRISPR data I encountered yours. I'm not sure though, if this can do what we need.
In our CRISPR run, we have our guides in the R2 file of the pair, so…
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Hi @ksahlin. Thanks a ton for all of your tools with noisy reads. I'm looking for a solution for de novo clustering of ONT amplicon reads from environmental sequencing, ie fungal rRNA amplicons. The…
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I have installed HlaTools sucessfully. But I don't know how to construct config file and input file. Can you give me an example? Thank you very much!
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External workflows, e.g. the once from the training material, should be updated regularly. Or even better should be a linked against the training material. Maybe we can pull them down before running t…