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I am working on a CRISPR data treated by two sgRNAs and sequenced with targeted sequencing. The distance between the two sgRNA is 3000bp which is much longer than amplicon. The targeted area is the w…
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hi,how to resolve this problem ?
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Writing to output...
Primers combination number 1
Number of written amplicons: 272
Sorting amplicons to multiplexes 1_2 (…
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Hi Author(s),
I think it is super cool that you've implemented this dynamic strategy!
We where trying to normalize some amplicons on a given chromosome, so we made sequences that where unique to…
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Options:
- [ ] Only Amplicons vs. semi-Amplicons: with only one primer hybridize due to partial sequences
- [ ] Min and max length of the amplicon
- [ ] Ta and max delta-G
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Hi,
I have a particular case and wanted to know If I can address this with `minimap2`. I have some Illumina reads that come from several amplicons and I also have a list of primers that generated …
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Hi,
In version 0.15.0 seqkit amplicon only keeps one amplicon (the largest) per primer pair. I don't always care about the largest amplicon.
Would it be possible to implement the feature of kee…
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Hi,
I would like to analyse multiple samples at once after demultiplexing. As input data I have one fasta file for each sample and each amplicon.
I'm not sure how to organise my data and I coul…
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Hello!
I'm experiencing some issues with RAMPART and I'm wondering if there is a solution available. I would like to know if anyone has been able to resolve similar problems.
In our laboratory, …
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Hello,
We are using CRISPResso2 pooled to map reads to multiple amplicons and it's working very nicely as long as the guide matches the amplicon perfectly. However, we also have cases where a guid…
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Dear Mr/Mrs,
I am trying to combine data from two PacBio runs: DNA (10kb) + cDNA (10kb), these regions overlap each other partially and I would like to phase them based on short-tandem repeats and …