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bcl2fastq uses "--no-lane-splitting" option. We need to check if need it for NextSeq
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Of the fourteen software, I requested ITS to install five are yet to be installed. These are :
1. LMAT (Livermore Metagenomics Analysis Toolkit): http://computation.llnl.gov/projects/lmat-livermor…
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Hi,
Below two cases is related to the downloaded sample sheet from Edit Runs
1) In the Edit Runs -> If we download the "Sample sheet -> BCL2FASTQ" excel file, as the sample are listing in rand…
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Hi, just came across digestiflow and it seems like an awesome solution for smaller sequencing groups! I am thinking of implementing it in my organization but for one thing - we are starting to process…
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As your neighbours at AGRF produce BCLs for CellRanger, it would be useful to integrate preprocessing from BCLs to fastq to make things more seamless and keep all analysis within a single framework.
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Hi There,
Thanks for samblaster, it's such a great tool. I wonder if you have considered supporting unique molecular identifiers at all? bcl2fastq supports adding them to the read name. So if a standa…
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Dear Xi,
many many thanks for this brilliant resource! I had one question: on https://teichlab.github.io/scg_lib_structs/methods_html/Illumina.html, the 'Truseq Read1' sequences under 'Truseq Sing…
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I'm trying to run the `asap_to_kite_v2.py` script on scADT-seq data only. After running bcl2fastq, I only have read1 and read2, but see references to a read3 in the code that is causing me some errors…
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Hi,
Thanks for developing fastp! I was wondering if it detects and removes PhiX spike-in by default?
Thanks in advance!
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Hello, I am trying to run the program and am coming across this error. Do you have any advice?
$ variant Sorted20130SAMy43Aligned.bam --motif Telomotifs.txt -b -o TelofromSorted.bam
...making the …