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Hi, I ran PASA with singularity to perform transcripts assembly and annotation update. The alignment assembly step ran successfully, but the update step ran with error, here are the error information:…
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I appreciate your developing a fast and good assembler and following up the issues here. We are currently working on animal genomes of >4Gb with high abundance (>60%) of repetitive elements.
Here …
sighe updated
3 years ago
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Generate test sets to evaluate assembler performance.
Therefore, use Arabidopsis genome and chloroplast from Genbank and simulate short read libraries fulfilling those characteristics:
1. Read l…
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Good afternoon,
I am assembling fish genomes de novo using hifi data and have run into a few issues for a few of my target species (all diploid);
first, to better understand the size and heterozyg…
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Hello I want to build my own database (GTDB + own built MAGs). I used prodigal to convert my nucleotide fasta files to protein fasta files.
As I see prodigal assigns as first column of a fasta heade…
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Hi @elimoss
I am using your tool for assembling my long reads metagenome. I have a doubt in the procedure of assembly that does the lathe also bin the genomes before doing the circularization or do w…
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there are three ways to do this.
* mapping based: map reads, call variants, build a variant call file (VCF), estimate ANI from VCF SNP calls.
* mapping based: create a new consensus genome based o…
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Hi, brianjohnhaas.
I found a small problem when using PASApipeline v2.5.2 (or PASApipeline v2.5.3) via singularity to update my genome annotation.
The log remained me that **fasta** and **pasa binar…
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## on which platform/server? (Windows? Windows Sublinux? MacOS? Ubuntu? etc.)
Linux
## MitoZ version?
3.6
## How did you install MitoZ? (e.g. Docker, Udocker, Singularity, Conda-Pack, Conda, o…
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Hi, there
I have obtained the EVM final results , but there are some problems that I am confused. Need help!
![image](https://user-images.githubusercontent.com/31943359/118354782-67c01080-b59f-11eb…