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Good morning,
I'm having an issue with alignment and subsequent sorting of files.
My pipeline is: sequencing with MinION (Nanopore), basecalling and demultiplexing with Guppy, alignment with NGM…
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Hi, thanks for developing the tool. I finish the procedure before freddie_isoform.py and ensure there were output in split, segment and cluster directions. However, when I processed the annotation the…
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Hi,
Thank you developing the those useful APIs for Miniknow. I have computer A connected with Minknow and a computer B used to process the data. I am interested in monitoring the sequencing status…
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Hi,
Thanks for developing this great software!
I have encountered an error in the polishing step when running Abruijn on a mixed/metagenomic 1D Nanopore-dataset (many organisms with varying cove…
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I had recently did a Nanopore sequencing run for SARS-CoV-2 in MinION with midnight primers 1200 bp amplicons (Nikki Freed protocol) and native barcoding kit (EXP-NBD96). I have earlier used EPI2ME pi…
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I recently started dipping my toes into published datasets from the Oxford Nanopore MinION sequencer. Their format is an hdf5 file, containing several types of metadata, "event data" (which is essenti…
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All: I am rather new to this group & would like to introduce open-source applications that I frequently use in our metagenome/metaproteome workflows (primarily on HPC systems):
00. Omega2: https…
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Hi
Any plans for expanding to include models for 6mA methylations?
Best regards
Rasmus
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To write this up we need to record runtimes for how long it takes (wall and processing time) to process a minion dataset, both for marginAlign and marginCaller.
I think it would be good to do this o…
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Hi, i am currently trying to trim a fastq file i got from publicly available datasets with Nanofilt.
I have the following codes:
[nanofilt_script.txt](https://github.com/user-attachments/files/160…