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### Operating System
Windows 10
### Other Linux
_No response_
### Workflow Version
v1.2.1
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow execution
_No resp…
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Maybe I missed something in the documentation, but I could not find anything about the --transcriptome-file file format. I tried a gtf file, but got segmentation fault and no output in the log files.
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Where to upload:
And what files to upload:
edsu7 updated
2 months ago
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Hi,
I'm using STAR to do an RNAseq analysis on Staphylococcus aureus sRNA.
The aligment is working, but during the maping I have this Fatal error:
Transcriptome.cpp:18:Transcriptome: exiting b…
Mpvrd updated
2 months ago
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Hi,
I ran mantee without specifying a transcriptome index, the program is supposed to create its own index but outputs the next message and dies instead:
"The provided transcriptome index does n…
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Hello,
I'm getting this error:
```
fish: Job 1, 'tools/immunopeptidogenomics/squ…' terminated by signal SIGBUS (Misaligned address error)
```
When running squish like so:
```sh
tools/immuno…
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I get the below error when running on a HPC with 120 cores and 500GB RAM. I read that 137 relates to memory so I increased the allocation to 250G using the -c parameter and a mem.config file, as sugge…
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Hi,
I've been trying out m6anet on our RNA004 reads and I have a question about alignment. As a default, we tend to align our reads to the genome for isoform discovery. From what I noticed in the d…
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What is the motivation for assembling the Transcriptome with trinity and translating that, rather than mapping the Transcriptome data to the assembly and building gene models that way?
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Hi @guokai8 , This is a very good tool for working with single-cell data. Will a tool to process spatial transcriptomes be considered later?