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Hello,
I'm using ITSxpress in combination with Nanopore sequencing reads, and find that only 4-6% of the sequenced reads are annotated as ITS reads.
We are having some issues with the PCR, so i…
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I am interested in duplex calling using Dorado but have a few questions that I can't find the answers to.
The Dorado readme describes a process to split pod5 files to enable distributed duplex call…
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Good evening,
We had samples from swine oral swabs that were sent to a core facility for sequencing. I am currently working with 16S V3-V4 amplicon sequences using 341F- 805R primers. Sequences w…
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> In fact, for three of these four animal coronaviruses, the strongest correlation between the number of viral and animal reads is for the animal species known to be infected with the virus...
The …
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Dear Prof
I trust you are keeping well
I want to know, that is it necessary to have phylogenetic tree for this model, or we can use this with out tree
kindly please let me know
Thanks and Regards
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Modulation of the Gut Microbiota in Memory Impairment and Alzheimer’s Disease via the Inhibition of the Parasympathetic Nervous System -- Park and Wu -- International Journal of Molecular Sciences
ht…
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### Description of the bug
Hi,
I had issues running a few samples through viralrecon 2.5 with ivar_variants_to_vcf.py so I decided to run it on the dev branch and it made it past those errors but …
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Using qiime2-amplicon-2024.2 with CLI
qc-rarefy errored on the first sample that is in the unrarefied and not in the rarefied dataset
Code:
```
qiime gemelli rpca \
--i-table ../data/core_d…
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Hello!
I want to use dada2 for creating an ASV table of my 16S V3-V4 sequencing data. Sequencing was performed with Illumina PE250.
The quality profiles of my sequences look (suspiciously) good s…
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Hi,
I have been wrangling some metabarcoding MiSeq data using dada2 and otu clustering to help simplify the search for recombinant sequences. I have managed to run through my make-shift workflow, …