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Hello,
I am attempting to utilize this program to analyze some amplicon data, but I'm having an issue parsing through this first few blocks of code because I don't need to convert anything from ab1…
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hello Developer,
Thank you for developing this tool.
I am not sure which module should I use for my purpose, we have crispr guided to a target region. we then sequenced the genomic dna (pcr p…
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Hi I am using crispr guided to a target region. we then sequenced the genomic dna (pcr product) that targets this region using miseq 2x250 + 10bp Index1 + 8bp Index2 (as defined in my previous questio…
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Hello! We've noticed that occasionally, guides that we score will get a *negative* predicted score from azimuth. Here's an example:
```
> from azimuth.model_comparison import predict
> import num…
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Hi,
First I would like to thank developer group for coming up with a convenient and easily usable tool. I would like to ask a question rather than a issue regarding the software.
**Background:**…
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Following these instructions: https://synbiodex.github.io/libSBOL/installation.html#build_from_source for Debian/Ubuntu. For linux, there's no pip install for pySBOL so I'm trying to build the python …
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When a crispr sequence has no matching sequences in search sequence, then nothing is stored in hash. This causes an "undefined value" error when sorting unique identities for each match.
- [x] The…
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Some parts of the [code example for constructing a CRISPR circuit PDF](http://sbolstandard.org/wp-content/uploads/2016/09/untitled-1.pdf) are unclear or don't cover steps that the user encounters in E…
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Hello,
I'm trying to plot InDels of a CRISPR experiment using CrispRVariants and I'm mostly successful. However, in the final plot the black window that indicates the guide position and cutting sit…