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Hello,
I'm trying to run a fasta file produced from the SQK-RLB001 kit and am getting nearly half of my sequences in the 'none' file, even after lowering the barcode threshold to 20. Is this someth…
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It seems that all demultiplexed output is gzip-compressed by default. Is there a way to turn off compression on these output files?
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Hi,
I am using racon, and get following error:
[01:45:17 main] Using PAF for input alignments. (/gpfs0/home/lzb0021/ouc/pacbio/ajap.paf)
[01:45:17 main] Loading reads.
ERROR: Reads are not speci…
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Hello Canu Gurus,
I am using Canu version 1.4 with the following command
```
~/software/canu/Linux-amd64/bin/canu -p fw205_1f31d2g3 -d fw205_1f31d2g3_minion genomeSize=165k minReadLength=100 minO…
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Hi,
Thank you for developing this fine tool.
Unfortunaley, we have been experiencing some problems with one of our assemblies. The dataset is about 5.0 Gbp of Oxford Nanopore 1D reads (from R9.5 por…
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Dear,
I was trying to assembly 274 reads from a nanopore minion run with canu using the following command line:
```
canu -p Canu_assembly -d Canu_assembly corMinCoverage=0 errorRate=0.035 contigFi…
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Hi,
I used Canu in the past and worked great! We recently did a migration to a new server (SGE) and I have been trying to run a job there, but it keeps stopping before the last step, failing to ali…
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### Subject of the issue
Hello Pierre ,
I want to use SamFixCigar on mapping data from MinION R9.4 datatsets (https://github.com/nanopore-wgs-consortium/NA12878), to get .sam files with a CIGAR no…
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I am testing using Canu with 25 Nanopore 1D reads. I am getting failure notices but I am not sure how to fix the error. Here is the code I ran:
```
#!/bin/bash
#PBS -l nodes=n03:ppn=8
#PBS -l …
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Hello Canu team,
I'm trying to run canu to assemble a 14 MB genome with MinIon reads.
It works find for a couple of samples, but in two of them I get this:
```
-- Canu release v1.5
-- Detect…