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Hi Simroux:
I really appreciated this pipeline you contributed to the viral study.
This is a question. In the predictions, the comments in the genebank file were inconsistent with the information in…
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Hello, thanks a lot for the great assembler.
I used hifiasm to assmbly three different heterozygous insect genomes using the option "-l 3". For the most heterozygous species I got a big circular sc…
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Generate test sets to evaluate assembler performance.
Therefore, use Arabidopsis genome and chloroplast from Genbank and simulate short read libraries fulfilling those characteristics:
1. Read l…
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Another idea is to buy a commercial S30-lysate kit to give this a spin on kinases before diving in to produce our own S30-lysate in large scale:
https://www.promega.com/products/protein-expression/pro…
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From Mike:
> Ok, so, here is the macro that OSU would like to run for ESELog. I think I can figure this out given what you did for us last time (with the updates you are making), but thought I woul…
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Hi Inigo,
‘’If there are the strong disagreements between the number of discordant reads and split reads the circular DNA should be handled with care. As an example, if a circular DNA contains tens…
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More a question :-)
I am running spades v13 on relatively simple plasmid mixture (two of few related plasmid backbones) using paired end read data and wonder wether I should run SPAdes in standard …
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Apparently the information can be read from SnapGene `.dna` files as xml. This would be important to do, since it will allow people familiar with SnapGenes' UI to do the cloning there, but still share…
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Hi, recently I was trying to use Circle-Map to analyze WGS data on GEO. And I got 20 "my_unknown_circle.bed" files following "Identification of circular DNA using Circle Map Realign" tutorial. The who…