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### Description of feature
Hello!
Thanks again for the excellent package; I've been using it lately with great success (and fun!). My feature request is for the ability to choose a subset of `var_…
hrj21 updated
1 month ago
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Hi,
I would like to apply CytoNorm to my mass-cytometry dataset but I would like to know more about the data used.
Do you use raw FCS files (after beads normalization and debarcoding) or do you "c…
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Hi,
I have data obtained from two different single-cell RNA-seq platforms:
* **icell8**: **specific cell type** obtained by flow cytometry
* **10x**: **all cell types except the above**
Now,…
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Hi, I have a question. fdaNorm was mentioned in the paper, 'Per-Channel Basis Normalization Methods for Flow Cytometry Data'. But i can't find the fdaNorm function in flowStats package. Has it been re…
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When I tried to run the example, it reported the following errors.
2022-09-07 21:04:53,032:INFO:91:root: Execution arguments and environment saved to "/tmp/cytokit-example/cellular-marker/20181116-…
d1015 updated
2 years ago
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Hi,
I 'm interested in the research "Breast cancer metastasis: immune profiling of lymph nodes reveals exhaustion of effector T cells and immunosuppression". But I can't find related code or data i…
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Hi!
I have been experimenting using Milo to analyse mass cytometry data. The data I try to feed into the Milo pipeline has been clustered using FlowSOM, and I am using UMAP for 2D projection. My SC…
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Currently the noise is applied to y values, and the amount of noise increases with y value. This makes the peaks look "realistic" but for low values of y the curve is still very smooth.
Suggestion: …
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Hi,
I am currently working with image analysis data. There I would love to be able to gate the cells as I could do in fcs. The problem is that they are not a .fcs file and I try to load it as a panda…
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Hi there,
It is Mario. I am doing a cytometry data analysis. But, when I do the compensation, this message appears in R:
> spillover(fcsfile)
Error in .local(x, ...) : No spillover matrix store…