-
I want to perform demultiplexing on paired-end fastq files but with barcodes for each pair fastq file.
This basic command works well
```
$flexbar -r $R1_fastq --barcodes $Tags
```
This one too…
-
I'm starting to regularly analyze samples multiplexed with HTOs a la [Stoeckius et al](https://www.biorxiv.org/content/early/2017/12/21/237693) and multimodal CITE-seq ADTs. I have some rough ports o…
-
Hi there!
I'm following the guidelines for the mixed oriented reads case, trying to demultiplex a bunch of 4 different files (x_R1.fastq.gz, x_R2.fastq.gz, y_R1.fastq.gz and y_R2.fastq.gz).
Afte…
-
Hi again, and thanks again for all the help with the great pipeline!
Now this error:
> ERROR ~ Error executing process > 'HADGE:run_multi:gene_demultiplexing:variant_freebayes:freebayes (sid456)'…
-
Hello,
I am the developer of demuxmix, a novel package for demultiplexing single-cell RNA sequencing data utilizing HTOs. I am curious if you would consider including it as an alternative to hashedDr…
-
Hi Kevin,
Many thanks for providing Axe to the community!
I've just come across your tool and would be interested to give it a try – however for my downstream applications it'd be rather essenti…
-
Hi Joe,
I just run across a `Segmentation fault.` error, when demultiplexing from barcodes in the header. However, all `%.fastq.gz` files are created as empty files, so the error must occur afterwa…
-
Hi,
I just discovered the tool and used it for de multiplexing of an Illumina MiSeq run, it seemed to work well! (only 6% of reads with unclear barcodes for 91 pooled samples).
On the same run …
-
Hi,
I am getting this error message, even after making sure, all the required packages are installed, the genome is well-indexed, the files are downloaded from SRA.
Command Used :
#############…
-
Index fasta file for demultiplexing produces an error when using mixed end of line styles: \r \n
Issue resolved by using windows-style at every line end.