-
作者您好，我最近用新版exomePeak2的时候报了这样的错：
Import genomic features from the file as a GRanges object ... OK
Prepare the 'metadata' data frame ... OK
Make the TxDb object ... OK
Extract bin features ... OK
C…
-
# Issue Report
unbelievably slow bascalling using dorado
## Please describe the issue:
I have sequenced some samples using direct RNA-seq on promethION machine. I've been running dorado for basecal…
-
Hello,
Thank you for developing modkit and being so responsive to community support.
I'm essentially wondering whether my direct RNA samples have any modifications that I can confidently infer …
-
Dear m6anet-team,
I have a question regarding m6anet, which I try to use in Galaxy to reproduce some RNA-sequencing study in order to get familiar with this topic.
In your manual you write for dat…
-
Hi！I have read your run_macs2.sh and have some question.
1. #transcriptome size estimate based on ucsc table browser summary for gencode v26 hg38 and mm10
For the macs2 readme documnent, only genome…
-
非常感谢作者开发出exomePeak2这么好用的包。我在使用过程中发现，一旦在计算三次重复的的输入值时，会出现奇怪的结果，比如
基本所有peak的padj值都为0.998， score值都等于0.000180458。 但是三次重复分开三次运行时，结果却是正常的。下面是我的运行代码，我现在很困惑，希望作者能够帮忙答疑解惑，非常感谢🙏🙏🙏
f1="CK1_IP_.sorted.bam"
f2=…
-
Hello!
Thanks for taking the time to read. I am the developer of [fibertools](https://github.com/fiberseq/fibertools-rs), our long-read framework for studying chromatin accessibility using Fiber-se…
-
Dear Miguel,
Hello, Miguel. Recently I asked you a question about how to analyze a library sequenced by smRNA-Seq Kit.
When I asked you a question, at first I thought my library is suitable for p…
-
# Issue Report
[error] Too few arguments
## Please describe the issue:
[2024-08-05 21:02:10.576] [info] Running: "basecaller" "-x" "cpu" "--modified-bases" "m6A" "--mm2-preset" "splice" "-k14" "-…
-
Hi,
i have done the partitions of the fastq file as it was not finishing for whole fastq in time.
so now i have eventalign.txt file for each part. I am using m6anet for pred the m6a sites.
wha…