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Related to https://github.com/HumanCellAtlas/hca-data-wrangling/issues/388
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Hello!
I am new to Picrust2.0 and I am trying to figure out how to use a customized database for my ASVs. We originally designed two consortia from two set of bacteria (consortia #1 and 2). Each co…
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Hi!
I'm working on 16S sequences from Illumina 2x300, with targeting regions V3-V4 (341F-785R)...
Forward:
Reverse:
These are the quality profile after cutadapt.
We were trying to targ…
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HI
I am trying to run the DADA2 pipeline for PacBio full length data. I am on the step where I define the path to remove primers and I am unable to select all the sequences to run in a single comma…
nhg12 updated
4 months ago
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Hi,
I am using the DADA2 outputs tutorial to analyze my data and I am having issue with the merging step.
The commands that I used are listed below:
# Load custom functions, set working director…
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Hello, we are running dorado on 2 samples. One native DNA vs the PCR amplified one that "in theory" should not hold the methylation.
Why do we get a methylation signal in the PCR sample?
The methyl…
BioRB updated
2 weeks ago
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Hello!
I am trying to use AmpliSIM as following but it gives an error "Error generating the amplicons." without any explanation of the issue.
`./amplisim ~/ref_seqs/NC_045512_Hu-1.fasta ~/ref_se…
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Hi @benjjneb,
Thank you so much for the incredible help you've been providing to everyone. I am right now looking for the best filtering parameters for my data and have been running some tests on …
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Hi I am doing meta-analysis using Dada2. The sequence files were from different primer region (eg. V3-V4, V5-V9), and different sequences platforms (Illumina, 454 and ion torrent). My questions is how…
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### Ask away!
Can you clarify if from the output of the pipeline what is in this sorted.aligned.bam file.
I am looking for an output bam file of the reads that aligned to the amplicon of interes…