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Hi! I am trying to use SIQ to analyse my Illumina data and majority of my reads appear in the category UnmergedCorrectPositionFR (for example in one of my runs, 81415 reads from a total of 123759). Th…
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I am trying to use cutadapt for the first time to trim 300 paired-end fastq files generated using 16S rRNA gene amplicon sequencing of the V3-V4 regions of the 16S rRNA gene. I am doing this analysis …
ghost updated
8 months ago
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Dear @dawnmy @alicemchardy @foobarx @fernandomeyer @TRKlingen . Many thanks for this wonderful tool. I have tried to get it to work for me for the first time and I get conflicting results.
I have thr…
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I downloaded the entire database from the site: http://mitofish.aori.u-tokyo.ac.jp/species/detail/download/?filename=download%2F/complete_partial_mitogenomes.zip
Then I used this command
```
$ ma…
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Hello,
First of all, I am a fan of Cutadapt, used and cited many many times!
However, I am having an issue right now I cannot actually understand why, I have several `fastq` files marker reads and…
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**Describe the bug**
Annotations are missing from some plots. Most noticeably, this occurs with allele frequency quilts. Only the colors for each nucleotide are visible, but not the letters. Sometime…
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Hi,
I am currently trying to basecall and demultiplex my reads which were sequenced using custom barcodes. So far I have tried a lot of different options for basecalling and demultiplexing using Dor…
bxm19 updated
6 months ago
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Hi
I have a small query. When I primarily process my sample using `wf-artic` pipeline and the upload consensus fasta on nextclade, I do not obtain any private mutations.
However, when I perform…
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Hello @benjjneb and DADA2 community!
I am having a very similar problem as issue #831 where I lose a considerable portion of my sequences during the merging step although I maintain a sufficient …
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Hello. I've successfully used MaAsLin2 to identify significant correlations between taxa and pathways across different groups.
Specifically, I'm interested in delving deeper into understanding whic…