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Hi @gjeunen,
I would like to extract the amplicon regions from the same database using the insilico_pcr and pga functions for a large number of primers. I tried setting up a SLURM job to do this, …
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I am extremely interested in using priors.
What is the format of this file?
fasta, amplicon style (for example, V4 region only)
or fasta full 16S read?
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Hi,
In version 0.15.0 seqkit amplicon only keeps one amplicon (the largest) per primer pair. I don't always care about the largest amplicon.
Would it be possible to implement the feature of kee…
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Hi,
We are experiencing error during the processing of file_0.todo. It says "OSError: [Errno 26] Text file busy". We are running in Ubuntu 22.04 virtual box. It seeems the error occurs when Thread…
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I am seeing intermittent cases where Trimmomatic is failing to remove adapter sequences properly. In the attached alignment, it is assembling 4 separate contigs from what is clearly the same VDJ rearr…
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Hello! Thanks for this great tool.
I'm using `dicey hunt` to check for uniqueness of primers in my target genome, and would like to do it in routine on a set of sequences, instead of one by one - c…
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Hi all,
I did an PE screen where I have more than one edit induced in the same sequence.
So this means I have one amplicon as reference but more than one pegRNA, pegRNA extension sequences and ngR…
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request from @bebatut
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Hi there,
I browsed your interesting [paper](https://doi.org/10.1093/nar/gkad158) (that isnt mentioned in the README) and was intrigued by the speed improvement while promising a similar performanc…
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Hello,
Thank you for this great tool!
I'm working with amplicon sequencing data capturing SMN gene. However, my data demonstrate uneven coverage along the SMN1 gene, which has resulted in question…