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I have a custom MultiQC module called `target_phasing`, which can be found [here](https://github.com/lumc/multiqc_pgx). It runs in MultiQC v1.11 and before, but not in MultiQC v1.12 or later. The modu…
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If fastq files are input into bc_demultiplex.py that contain an underscore in the sample id, all reads will be assigned to undetermined, since the "lane" field (as set on line 49) will not match the l…
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## Documentation request
### Tool(s) involved
ExtractIlluminaBarcodes/IlluminaBasecallsToSam
### Description
Hi,
Is there any history of anyone demultiplexing S4 chips using `IlluminaBasecal…
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Can we add a QC step that verifies there is the same number of forward and reverse reads?
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## Bug report
I observed unusual behavior in Nextflow with Docker enabled, despite the .command.sh file appearing correct:
```
#!/bin/bash -eo pipefail
bcl2fastq --runfolder-dir bclFolder --p…
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Some papers use STAR with parameters TK .
But i cannt find the paremeter, can you tell me the function of "TK"
Thanks!
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Hi,
I have cite-seq data with only one protein. After running cite-seq-count and use Read10x() to read the umi_count matrices, I noticed that my matrix is very sparse. Since I only have 1 protein,…
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https://blog.software.illumina.com/2020/07/30/announcing-the-release-of-bcl-convert-software/
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Hi lovely people from dopEst.
I was wondering the feature for running dopest on indrop v3 on specific library tags was tested more thoroughly and is indeed functional? In the manual you specify:
"…