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https://mp.weixin.qq.com/s/q0sdf_0mEkP_VeR4Wqbvew
ixxmu updated
1 month ago
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Hello,
I am using m6ANet to detect m6A modifications in RNA and recently swapped over to the new nanopore RNA004 chemistry/kit. Because of this, my pipeline uses ONT's new basecaller, dorado, and I…
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#### Summary
Node stuck in "Generating state snapshot" and not processing new blocks after upgrade to the latest software releases of op-geth (v1.101315.0 -> v1.101315.2) and op-node (v1.7.5 -> v1.7.…
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Hello! I've been learning to use dorado and modkit to process my direct-RNA seq data for m6A modifications for the last couple days, and I want to make sure I understand the internal logic.
First I…
mbosm updated
7 months ago
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Hi,
I keep getting an error message and empty output files (data.index, data.json, data.log, data.readcount eventalign.index) when running the xpore dataprep step for genomic coordinates.
I have …
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Hi,
I am trying to run eventalign on reads from RNA004 and I get the following error:
[slow5_idx_init::INFO][1;34m Index file not found. Creating an index at '/path_to_/sample.blow5.idx'.[0m
[s…
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https://mp.weixin.qq.com/s/5ka15kregXe4XDpLyW-Z-w
ixxmu updated
4 months ago
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https://mp.weixin.qq.com/s/v7HJ0AWW-IEhArJREkSuIw
ixxmu updated
8 months ago
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https://mp.weixin.qq.com/s/nJJrH46Vg1a4v4vYVK0RhA
ixxmu updated
4 months ago
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Dear all,
I am kind of new to analyzing epigenetics data so you have to excuse my naive question:
When I use Dorado for basecalling m6A-RNA modifications from direct RNA-sequencing and the resulti…