-
Dear prof. Heng Li,
Thank you so much for the tool! I have been using hifiasm since 2021, and am a big fan of it. I recently run into different results from different versions of hifiasm, and would…
-
Hi,
Due to the nature of our system we are unable to acquire the parents of our sequenced individual, we sequencing a lot of children data.
What should I do to build hap-kmer using these children…
-
Dear author
I would like to consult where the color is inconsistent in the picture, (the changing contig),What is the generating file that lets me know which contig it is?
![image](https://user-imag…
-
Hi,
There is huge difference with the haplotype 1 and 2 output with version.
Hifiasm version _0.15.4-r347** :
hifiasm -o xx.asm --primary--n-perturb 20000 --f-perturb 0.15 --seed 11 -l3 --n-wei…
-
Dear Haoyu,
I have run both one of the newest versions (**0.19.3-r572**) and the last fully stable version (**0.16.1-r375**) of Hifiasm with HiC-phasing, using default parameters.
I have assembl…
-
Good Morning
We trie to use your version of MultiQC on Galaxy with pangolin.
We seen that MultQC v 1.11 support pangolin.
But when we want to use it on Galaxy, we seem to have a problem for pan…
-
Hi again Dr. Rhie,
I'm having a great time using merqury to asses quality and completeness of different versions of our assembly. It's an awesome tool and it is allowing us to make more informed dec…
-
# Functionalities and modules
## Mentioned in the pipeline proposal
[primaryQC_pipeline_proposal.pdf](https://github.com/NationalGenomicsInfrastructure/seqinspector/files/14651445/primaryQC_pipe…
-
Hello, I have successfully completed the assembly, but now I want to evaluate the quality of the genome assembly. The software I want to use at present is Busco. But how do I convert this GFA format f…
-
Hi!! :)
I'm trying to run merqury but I can't obtain all the results. Could you help me please?
I'm following the steps:
# Step 1. Create meryl db:
`/opt/merqury-1.3/meryl count k=21 output /path…