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Hi! This is a really exciting looking piece of software - many thanks for developing it, I'm very interested in giving it a try.
I have a large amplicon sequencing dataset of 1,050 samples not incl…
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If I remember correctly, we agreed that experimental protocols using
* internal barcoding (i.e. not resolved by the sequencer) that is used for
* multiplexing at or above the cell level (i.e. not UM…
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Hi,
Thanks for making this software available to the community.
I've done several rounds of AA (using different seed intervals) and it usually runs successfully.
In a recent run, some of the sa…
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After the editing with two guides, I got two alleles, one long like the WT and a shorter one. For crispresso2 one has the expected deletion between the guides and one has an insertion (how can be if I…
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I inherited amplicon sequencing data on wild vs managed cranberry bogs and am using picrust2’s predictions to make hypotheses for a future shotgun sequencing experiment.
I was surprised to not see …
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/seandavi/MicroBioMap
Confirm the following by edi…
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Hi Jiru,
I've been helping Jason to troubleshoot why he's not been able to run AmpSeqR on his amplicon sequencing data and I *think* I've pinpointed more or less what happens. To give some computat…
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Hello,
I'm part of a project called the Common Fund Data Ecosystem (CFDE) (https://commonfund.nih.gov/dataecosystem). Our group, under PI Owen White, are the CFDE Coordinating Center. Among other…
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Hi,
I'm facing a curious problem. I've sequenced twice a sample : first with the R9 flowcell version and second with the R10 flowcell version, and it looks like I'm loosing some information in the…
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**Describe the bug**
Hi, I am trying to run crispressoPooled on a fastq file with 50 million reads and the job keeps getting killed for high memory usage. I gave the job 96G of memory and it seems to…