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My lab has been using the cual-id system for our barcodes for 2 large projects. It has worked great for us. Having made over 800 barcodes and working with them on a daily basis, we have noticed 2 thin…
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Hi,
I am using AmpBinner to demultiplex Nanopore sequencing data generated from the 10X V3 library.
The cell barcode assignment rate is approximately 20%. Do you know how to improve it?
Thanks!
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Hi, I have some unpaired scRNA-seq and scATAC-seq data, they are sequencing the same samples, but in separate scRNA-seq and scATAC-seq assays, so the cell barcodes are different in these datasets. How…
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Dear Developers,
Thanks for introducing such a tool to detect multiplets in single-cell epigenomic data.
I’m completely new to using this tool and have some simple questions. I have successfully…
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Hi,
I have run the [epi2me-labs/wf-single-cell](https://github.com/epi2me-labs/wf-single-cell/tree/master) workflow on my nanopore data but would like to create consensus sequences for reads tagge…
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### Is your feature related to a problem?
In one of my samples, wf-single-cell fails to correctly find the cutoff in the knee plot and returns an excessive large number of cells. Consequently, when l…
ddiez updated
1 month ago
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Hi again @drneavin :)
I got the error I was having on AssignCellsToSamples fixed yesterday. However, I'm now having a different error when running the DetectDoublets step of Dropulation. It seems …
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# Issue Report
## Please describe the issue:
Hi, I was testing different configurations to demux non-ONT barcodes in order to find optimal scoring and window sizes settings, and found inconsiste…
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Minimap2/Samtools is throwing an error from reads with append cell barcode/UMI (generated from `match_cell_barcode`).
```
[E::sam_parse1] query name too long
[W::sam_read1_sam] Parse error at lin…
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I encountered the following problems when running. What do you think is the cause of the problem? Thank you very much.
```
res = do_pipeline(
+ out_path ="...",
+ counts_file_dir = …
wyt14 updated
3 months ago