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Hi there,
I have two questions:
1. You have mentioned that "ONTbarcoder has been optimized for protein coding gene like COI. While **there are ways** to obtain consensus for length variable non …
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I have generated consensus sequences for different datasets using C3POa. I am trying to do some stats by stablishing a correlation between the number of subreads and the accuracy of the consensus. Wh…
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Hi,
I'm having difficulty clustering using profiles when following the instructions in the wiki. Specifically I'm referring to this section:
```
# extract consensus sequences from profiles
…
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I have observed cases where my dual UMIs can get swapped during amplification steps in library prep, resulting in two consensus sequences which align to the same start/stop position but share the half…
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Hi Saskia and thank you for stiching up this workflow,
I am encountering a problem when wanting to run blast option. I did it on the `example_data` as well, but there is no output from blast to be …
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Hi,
I am using version 1.3.2 to polish 2 sequences (27903 bp and 4492 bp long) with 835 nanopore reads, but I encountered a segfault after consensus has completed according to the log.
```
[racon…
kmnip updated
5 years ago
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Hi,
I'm using MEME and FUBAR in a dataset of 6 species, but I'm most interested only in one. I'm wondering if for that species I should include all the individual's sequences that I have or just a…
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`assign_taxonomy.py -m uclust` (the new consensus-based taxonomy assigner) should throw an error (or at least a warning) if aligned sequences are supplied via `-i`.
This issue [recently came up](http…
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I.e. A 3k consensus sequence that could contain
"AGGGGGGGGA" or
"AGGGGGGGA"
but would otherwise be identical.
Current spec does not seem sufficient since it appears to allow only 1-2 sequences, wher…
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Hello,
My question has two parts
**1.When I run the command**
`racon -m 8 -x -6 -g -8 -w 500 cluster_1329.fasta cluster_1329_ovlp_mapping_test_fwd.paf TAIR10_chr_all.fasta > cluster_1329_tmp…