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### Description of feature
ONT now has a faster tool than `guppy` or `bonito` for basecalling called [`dorado`](https://github.com/nanoporetech/dorado). It is pretty stable and mature now. Please con…
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Hi,
I was just processing a bunch of reads with dorado trim (0.7.3) and noticed that it keeps saying:
`[info] > Simplex reads basecalled: 456331`
And if I turn verbose on:
```
[debug] Total…
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- Update Dorado v0.5.3 --> v0.7.X
- Download latest models (or at least v3 = as ND uses them)
- Add `dorado trim` rule
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Hi, taiyaki provided `calibrate_qscores_byread.py` that estimated calibration variables.
How to estimate `scale` and `bias` for dorado models?
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I recently did a run with the following configuration and output:
I base called and demultiplexed this data using the following script run on a Linux-based cluster:
```
module load …
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# Issue Report
## Please describe the issue:
Multiple compiler warnings being treated as errors are resulting in a failure to build from source, unless `-Wno-error` is used:
```
/tmp/easybui…
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Dear m6anet-team,
I have a question regarding m6anet, which I try to use in Galaxy to reproduce some RNA-sequencing study in order to get familiar with this topic.
In your manual you write for dat…
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Hi ,
I re-basecalled two control sequences containing 100 % and 0 % pseU modified U's at one position, respectively, using the latest dorado basecaller (v0.7.0) and pseU modification calling (rna0…
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Hi,
Is it possible or would it be possible for dorado to handle a sample sheet across multiple positions/flow cells, essentially allowing the same sample or barcoded sample to be loaded across multip…
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Hi there,
Here is the whole process I run after dorado 5mC and 5hmC basecalling.
```
dorado aligner reference.fasta dorado_5mC_5hmC.bam > aligned.bam
samtools sort aligned.bam > aligned_sort.bam…