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Hi,
For a high heter rate (> 2%) plant genome, I got a hybrid assembly (BUSCO C ~84% D ~26%) and a ONT(~30X) alone assembly (BUSCO C ~80% D ~14%).
Should I need to remove redundance sequences be…
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I have two long read assemblies that are better than the one in `003_long_read_assembly.gfa`: `minimap2` with `miniasm -c2` and Racon twice (good) and Canu (best).
> Added `--existing_long_read_as…
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Dear all,
I recently tested scripts in 4-Assembly folder and found what needs to be fixed.
1. runpipeline.sh
cp $core_genome ${output_dir}/core_assembly/step0_assembly/ .
core_genome_file=${ou…
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Hi Kraken2 developers/community,
I recently built a large Kraken2 database with genomes from the NCBI RefSeq database. I added genomes regardless of assembly level and limited it to 1 assembly per …
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Which is first on the command line?
This may sound trivial, but to someone like me who is trying to figure out which of my assemblies I should give first on the command line and which second, it is…
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Hi,
I try to run unicycler for a hybrid assembly and when I arrive to the step "Aligning read" after the step "SPAdes assemblies", the run is stuck to 100% :
Aligning reads (2018-09-06 18:17:33…
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**Describe the bug**
Unexpected token . in Blazor Webview I'm encountering a SyntaxError: Unexpected token . in my Blazor Webview application. The error details are as follows:
```lua
blazor.webv…
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你好,我用如下命令跑:centromics -l ~/wang/cleanData/singleCell/hybrid/pacbioHifi/AZ-2.hifi_reads.fq.gz -g ../AZ-2hap2chr18.fa, 出现了错误,请问我该怎么解决呢?谢谢!
23-08-31 10:21:19 [INFO] run CMD: `blastn -query ../AZ-2hap2ch…
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Hi Mahul !
I have encountered some issues trying to run quickmerge within my conda environement.
I have installed quickmerge using the following :
`conda install -c conda-forge -c bioconda q…
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I have two batches of E.coli strains (individual colony isolates, illumina sequencing) that are very large (~1500 and ~1900 samples) and I want to use Unicycler hybrid assembly first, but if it fails,…