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I just came across the preprint and got curious to try out burst.
I need to assigning the taxonomy for Illumina short-reads (MiSeq, up to ~450bp) from an amplicon sequencing run (COI & 16S). Are t…
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Feedback from user looking for information on PCR genotyping. Asked for primary sequencies, PCR conditions and confirmation on whehter sequencing had been done as part of validation prior to phenotypi…
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I am mapping some FASTQs using a built-from-source dragen-os on:
wget "ftp://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/p13/hg38.p13.fa.gz" with MD5
9930e3071308dc5e9b546934b43a2323 hg38.p13…
biork updated
2 years ago
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Dear Mr Dierckxsens,
I am using NovoPlasty to assemble insect mitochondrial genome. I am wondering if we can identify contamination (for example during the DNA purification)?
I used one of the ass…
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@nbokulich, @mikerobeson, @BenKaehler - how important do you all think the region-specific classifiers are? My impression is that they offer fairly small classification performance gains relative to t…
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Some ideas from a chat with Fred Jaya. Use the wiki to give a few examples of simple benchmarks, e.g.
1. RaxML vs. IQ-TREE on individual loci (fix models to be equal, assess trees in one piece of s…
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- [ ] 1. genes ex. unc-13, gadr-5, par-2 regex ([a-z]{1,4}-\d+) italicized
- [ ] 2. proteins ex. UNC-13, GADR-5, PAR-2 regex ([A-Z]{1,4}-\d+) NOT italicized
- [ ] 3. variations ex. zu405, ox41,…
kyook updated
3 years ago
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* **PTHR ID & PTN node:**
* PTN000186700 - PTN000186702
* ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER (PTHR11670) - ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER (PTHR11670)
* **Sequences w…
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I'm having this issue as well. I've updated my gcc (gcc (GCC) 10.3.0), reinstalled sift4g, and have made sure that I have loaded the updated gcc version before running, but it still does not create a …
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Dear autor:
I am assembling a plant genome, and now it is assembled to the level without gap. It have 21 chromosomes, but so far only 39 have been counted, and there are 3 motifs (AAACCCT) w…