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I am interested in calling all the mismatches/conversions in my RNA modification dataset that is generated using Illumina TruSeq protocol (dUTP). I am using the JACUSA2 pileup method for this step. Si…
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Things that would need to be changed
- change spade's `--meta` to `--rna`
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After successfully installing RF2NA in my linux workstation, I tried to run these commands
```
# run Protein/RNA prediction
../run_RF2NA.sh rna_pred rna_binding_protein.fa R:RNA.fa
# run Protein…
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- [ ] Low hanging fruit: difference in length distributions (compare with human, mouse, amphioxus non-mapping transcripts)
- [ ] Folding structure for mapping vs non-mapping RNAs (or better, putative …
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while processing the data with Tombo before using MINES, which reference fasta should be used for ONT direct RNA sequencing data - genome or cDNA fasta.
akk01 updated
3 years ago
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Hi, Dengfeng
`purge_dups` is super easy to easy tool. Thanks for developing.
I use the Falcon-Unzip to assemble a outbreed plant (270M genome size, 1.8% het ,based on GenomeScope), but the Unzip…
baozg updated
4 years ago
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Hello, Dr. He. Sorry to bother you again.
I already knew that SEVtras would work with single cell RNA sequencing data produced by the 10x genomics chromium , which is based on droplet sequencing, but…
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# General Notes
This repository serves as a mostly automa…
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Some questions
* Will FACS sorting disturb RNA?
* How should we preserve the cells post-sorting and pre-RNA isolation?
* How should we isolate RNA?
* What markers should we sort on?
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when I run the step:
query