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I had recently did a Nanopore sequencing run for SARS-CoV-2 in MinION with midnight primers 1200 bp amplicons (Nikki Freed protocol) and native barcoding kit (EXP-NBD96). I have earlier used EPI2ME pi…
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### Description of the bug
Hi, there has been a new primer set version uploaded to https://github.com/artic-network/artic-ncov2019/tree/master/primer_schemes/nCoV-2019/V5.3.2, would it be possible to…
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Hi, firstly thank you for creating the nabseq_nf pipeline.
I've been running the nabseq pipeline on Seqera/Tower and noticed that the biggest bottleneck is the `subset_aligned_reads ` step, which f…
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I receive the following error message when when I try to run TEdenovo on my assembly
```
Fatal error: Exit code 1 ()
cat: /home/jeremy/galaxy/tools/Pipeline/REPET/WORK/genome_Blaster_Grouper_Map/…
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Hi.
I am having troubles getting epi2me-labs/wf-isofom to work with my ONT RNA data. In my mot recent attempt, I am using the following command:
```
OUTPUT=./output;
nextflow run epi2me-lab…
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Hi dear DROP team !
We are exploring possibilities to get into aberrant splicing analysis using long read RNA/cDNA sequencing from Nanopore sequencers.
Do you think it is achievable to use the D…
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### Operating System
Other Linux (please specify below)
### Other Linux
Red Hat Enterprise Linux 8.9
### Workflow Version
v2.2.0
### Workflow Execution
Command line (Cluster)
### Other workflo…
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My own analysis of Illumina (`SRR11140750`, bottom track) and nanopore (`SRR11140751`, top track) data from the same swab sample shows your variant analysis doesn't include indels:
. I used ont_fast5_api to demultiplex the multi-fast5s and then ran poreplex on those fast5s. Any input would be helpful. Thank you.
2019-01-31 14:21:01,777 Com…
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Hi
At the last step of Griffin, I used griffin_merge_sites.py to merge TFBSs for each TF, When I used top 1k sites, everything worked fine, but when I switched to top 10k TFBSs, this script seemed …