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### Ask away!
Hello!
[nextflow.log](https://github.com/epi2me-labs/wf-human-variation/files/14007577/nextflow.log)
Human-variation-workflow has stopped with an error exit status(137)
I guess i…
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I'm getting this error (see below). I used ont_fast5_api to demultiplex the multi-fast5s and then ran poreplex on those fast5s. Any input would be helpful. Thank you.
2019-01-31 14:21:01,777 Com…
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Good morning,
I'm trying to run Circuit-seq using the example data before trying it with our own, and I'm having a problem that I can't solve.
Everytime I run `bash Circuitseq/pipelines/run_nf.s…
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Hello,
The vcf that is outputted by pandora gives variant positions with respect to each individual gene. Is it possible to map the position of variants with respect to the pangenome reference?
…
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### Ask away!
Hi all,
I wrote my script but when I launch it it crashes without any error.
This is the command I run:
```
REFGEN=/data/references/genomics/hs37d5.decoy.fa
REFTRAN=/data2/refe…
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Can this pipeline also demultiplex reads from cell barcodes?
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Hi
When performing the analysis of finding m6A in DNA using alternative model with the default values the methylation rates are too high, for the control ones which were pcr products causing misstru…
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Hi
From #14 It seems that MotifSeq could demultiplex a bundle of fast5 to retrieve the fast5 for each strains. Is this right? Or there is some options in Squiggle Kit to do the same?
Thanks
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### Ask away!
Dear developer,
We are trying to use your wf-amplicon workflow on Epi2ME platform to quantify variants in amplicon libraries generated from the bacteria Desulfovibrio vulgaris. We ar…
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Hello,
I apologise if this is not the right location to make this type of enquiry. I am completing my master's in diagnostics genomics and I have some concerns and questions that I wanted to reach …