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To whom it may concern,
I am writing to inquire whether the authors of the program had compared the results from winnowmap -ax map-pb and -ax asm20 bam file.
Minimap2 recommends using asm20 for…
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@ekg
Hi Erik,
As you suggested, I tried different parameter values for PacBio data, something similar to BWA mem.
When I used
```
vg map -k 17 -W 40 -r 10 -q 2 -z 1 -o 1 -y 1 -x yeast.illu…
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The LRAS manuscript reproducible markdowns are fine, but a dedicated tutorial workflow is much preferred.
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Hi! I´m trying tu run Masurca with PacBio and Nanopore reads. I read that it is possible to supply them all as NANOPORE type. How do you add more than one file of Nanopore reads? I tried this:
NANO…
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Hi, I'm trying to run Braker to predict genes, but I'm getting this error with gmes_petap.pl script. When I try to look out on 'errors' folder and GeneMark-EP.stderr file is empty, so I can't find wha…
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## Merging reads per hostplant
As a comparative measure against population based approach, we will assemble metagenomes using all CH and CO samples separately.
```
# New directory for hostplant …
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Hello,
Thanks for developing such a great tool. I am using IsoQuant to analyze ONT single-cell full-length transcriptome data and would like to obtain exon count information to calculate PSI. In th…
weib3 updated
2 weeks ago
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1. SMART link user guide: https://www.pacb.com/wp-content/uploads/SMRT_Link_User_Guide_v10.1.pdf
2. Pacific Biosciences Glossary of Terms: https://www.pacb.com/wp-content/uploads/2015/09/Pacific-Bios…
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Hi all,
I've been trying to run HINGE using Circular Consensus Sequencing reads that are obtained after a Sequel run. As expected the number of reads decreases drastically due to CCS, but on the ot…
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Hello
I need some suggestions in simulating complex structural variants in Hifi reads file from PacBio
https://downloads.pacbcloud.com/public/dataset/HG002-CpG-methylation-202202/m64011_190830_22…