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I'm experiencing a weird thing when I use filterandtrim on some samples from a recent run, I haven't experienced it before, I've put two examples in here.
There are>100.000 reads for almost all sa…
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Is there documentation or guidance available for choosing the correct medaka model? We are currently using guppy version 6.4.6+ae70e8f and are updating to the new 10.4 chemistry and are wondering what…
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Hi,
gemBS seems to be a very interesting toolkit. My wet lab colleagues use targeted approach - they amplify specific regions of bisulfite converted DNA and analyze it using amplicon sequening on I…
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Hi,
thanks for this.
I have a lot of problems with incorrect and weird samplesheets from the lab generated with "copy-paste" and strange barcode schemes, such as mixed Truseq and Nextera.
I w…
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Hi Rasmus, have you had time to look at the amplicon mode i.e. FASTA in and FASTQ out with exactly the same sequences?
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Hello,
I am trying to analyze the humann output files to obtain the differentially abundant pathways in each group using aldex2_kw.
however I got this error
Error in aldex.clr.function(reads, con…
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Hi, I have problem in getting desired levels of a factor in facet_wrap of rarefaction curve even after i changed the levels of factor in dataset itself, the code is following,
##upload the required d…
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Hello, I want to make pcr primers for a several different genomes to make PCR in one pool. Maybe I am a little bit stupid, but I dont understand, is there a way how to do it with your program? Will NG…
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Hi,
I am trying to read from draft file and trying to make multiplex primer with -blast option but failed in checking for checking non specific region step, I have pasted error message below, please …
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Hi Michael,
Thanks for developing and maintaining Olivar. You might remember that we spoke a while ago about BED support for Olivar to help with assembler compatibility. I see you've since added this…