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Bug report by @irosenboom
It was reported for 2x150bp Novaseq reads that bwa mem performed alignments with extensive softclipping.
For example, some alignments were of 18bp with 82 bp simply so…
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sivico26 commented on May 20:
The command line I used to run Ra was the following:
ra -t $threads -x ont $ont_reads reads/ngs.fastq.gz > assembly.fasta
where $threads was set to 24 and $ont_reads, …
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Hi Andre and the Bambu team,
I am encountering an issue related to issue #444 . I merged read classes across 44 samples as you had suggested and found that there were about 1000 novel transcript is…
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Hello Developers of Gerbil,
I'm trying to install Gerbil in my desktop computer which has got Ubuntu 16.04, i7 processor and with GeForce GTX TITAN Black/PCI…
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conda is leveraged for installing (some) dependencies (at least, the recommended install method), but conda is not fully leveraged via a bioconda recipe for installing scNanoGPS.
Are there plans on…
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Hi Raymond,
Thanks so much for providing this tool. It worked great.
I was wondering if the same could be done for long reads, more specifically Oxford Nanopore?
Thanks :)
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Hi,
I have pacbio Hifi read assembly using Hifiasm. Hifiasm gave me much less fragmented assembly as compared to other tools. However, there are more contigs. Some of the them are quite small and…
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### Operating System
Other Linux (please specify below)
### Other Linux
Ubuntu
### Workflow Version
wf-human-variation v2.3.1-g6a6daa6
### Workflow Execution
Command line (Local)
### Other wor…
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### Ask away!
Hello, I would like to ask the following question:
We use **a novel 3' library preparation method** different from 10X (the main difference is that the barcode length of 10X v3 3prim…
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Hi Gao,
I tried to retrieve the repeated subunits from the long read and feed it into other consensus calling methods (such as Medaka by ONT or majority voting).
According to the README:
subPos:…
zztin updated
4 years ago