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Hi!
I have rRNA depleted total RNA-Seq reads obtained using below kits. Can I use these to identify all AS events? (I am getting SEs and MXEs and no other as events using rMATS.) Is there any speci…
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```
Hi,
I use cutadapt to trim Illumina reads for adaptor sequences and low-quality
bases. I would love to also have the option to filter out reads that fail the
Casava 1.8 filter ("Y" in sequence i…
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Basically I have a somewhat plausible long read assembly with several tandem repeats. When feeding it to Unicycler w/ `--existing_long_read_assembly` together w/ Illumina short reads, the assembly get…
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Maybe based on Unicycler after mapping reads to reference
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Hi,
Python 2 has been End of Life for over four years now. Are there plans on updating strelka2 to utilize python3 or should it be considered abandonware at this point?
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Hi,
I am using bowtie 2.3.5.1 and try to get sample and other IDs recorded on the `@RG` line. Unfortunately, it seems bowtie2 does not write TABs as separators on the `@RG` line and also copies who…
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Hi Dariober
when I run with unmatched mode (the unmatched bam are merged from 10 un-related normal samples)
cnv_facets.R \
-t ./bams/S21-096.bam \
-n merged_normal.bam \
--unmatched \
-vcf v…
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> [smoove] 2022/04/27 15:54:53 starting with version 0.2.8
> panic: sam: duplicate program name: line 3430: "@PG\tID:SAMBLASTER\tVN:0.1.24\tCL:samblaster -i dummy.cram.readsorted.sam -o dummy.cram.sa…
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```
Hi
What steps will reproduce the problem?
I am trying to generate the synchronized files with the two options which are
described in the manual (java and perl), I am expecting two columns with…
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We need to do a 'true' power analysis for readcomb - we know it detects phase changes, but how often do we expect it to actually catch them in real data?
We've looked into [ART](https://academic.o…