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It is very useful to know Ori/Ter in draft genomes.
However, is there a way to reorder multiple non-overlapping contigs then, such as from a WGS Illumina sequencing experiment yielding a 30-100 con…
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Heyhey, could you add the following biography to the website? I've also attached my picture to the post.
Thanks a lot!
### Lancelot Seillier, M.Sc.
Scientific Researcher / Bioinformatician
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Dear rMATs team,
I used rMATS-turbo using FASTQ files for the detection of differential alternative splicing. The FASTQ file had the standard Illumina adaptor for paired-end sequencing. I did not t…
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I am using "MarkDuplicates" (2.18.2.2) to remove duplicates from my sequencing datasets coming from NovaSeq. From Picard documentation it suggests the OPTICAL_DUPLICATE_PIXEL_DISTANCE to be 2500 for …
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Hi,
This issue isn't really an issue per se, but more a question about the status of `lofreq3`.
I use `lofreq` version: 2.1.5 in an analysis pipeline for SARS-CoV-2. I like updating to the lat…
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I am in the process of determining whether sourmash is the right tool for my metagenomic analyses. I am mostly using ONT data at the moment. Similar to previous posts (e.g. https://github.com/sourmash…
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```
What steps will reproduce the problem?
1.MosaikBuild -q 0019.fq -out 19.bin -st illumina
2.MosaikBuild -assignQual 10 -q 0019.fq -out 19.bin -st illumina
3.
What is the expected output?
---…
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I just came across the preprint and got curious to try out burst.
I need to assigning the taxonomy for Illumina short-reads (MiSeq, up to ~450bp) from an amplicon sequencing run (COI & 16S). Are t…
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Sometimes I just want to trim "from xx bases onward" or "take just the first xx bases". In trimmomatic (considerably slower than atropos) this is obtained with the `HEADCROP` and `CROP` commands, resp…