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Is there any scope for updating the primer3 version in primer3-py?
The 2.6.0 version adds an internal oligo parameter - SEQUENCE_INTERNAL_OVERLAP_JUNCTION_LIST which would be very useful for probe …
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Dear UniverSC developers and community,
I am running UniverSC for bd-rhapsody fastqs (cellranger 3.0.2. installed) and the run goes smoothly but there seems to be no barcode match:
**whitelist s…
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I have a problem about high through put PCR process, when template sequences may not be in fixed sequences, and primer oligos may occasionally bind to an unexpected position for some of our samples in…
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I have run the mipgen_practice with the example commands but all of the logistic scores are '-nan'. Is there a fix for this?
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Hi,
I am so sorry but I want to ask about CHiCAGO after HiCUP analyzing.
I want to make rmap and baitmap as follows command by chicagotools;
create_baitmap_rmap.pl
and then,
Specify i) digest …
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@sminot pointed out an error occurring when running the virscan library. Unfortunately, this error is slightly more nuanced than a simple formatting error.
```
Running phipflow
Traceback (most re…
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Hello,
I have a few questions that are not really related to each other.
First: I always assumed when training and running the model on new data, you had to know the reference in each case in or…
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Hi,
I've noticed some differences in the oligonucleotide sequences for SPLiT-seq between different protocol versions.
This is the structure of read2 based on the [preprint](https://www.biorxiv.o…
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I gave this the good ol' college try, but I just can't wrap my brain around the various ins and outs of our Typescript design. I know what to do from an HTTP point of view, but I just can't get my bra…
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Hi developers,
Thanks for developing this tool and useful resources. I checked the known probes provided by https://oligopaints.hms.harvard.edu/genome-files and found that only the genome sequence …