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Hello !
I encounter an error with your tool `Cluster, infer trajectories and embed with scanpy (Galaxy Version 1.4.4.post1+galaxy3)` when using it for the option `tl.paga`. I tried your test-data …
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I'm new to RNA velocyto and have a question on Velocyto
do we always have to go back to original raw file to estimate/calculate spliced and unspliced reads? or are there anyways that we can calcula…
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Dear Linnarsson lab,
I am quite new to programming, and only started working with single cell data in April 2020. I have a question and was hoping you would be willing to respond and help me pave m…
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When you input multiple FASTQ files into the `kb count` function, does it process them sequentially or is there a way to parallelize it? Especially because for me, the first step "kallisto bus" takes …
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Hi, when using the [usecase_rna_velocity](https://github.com/dynverse/dyngen_manuscript/blob/8370bf5ce8a4c9e78967122202ccfee56fd516f1/analysis/usecase_rna_velocity/1_datasets.R#L19)
```
pwalk(design…
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Hi,
I am running over the tutorial of 'RNA velocity with kallisto | bus and velocyto.R'.... and I have two lines that I can't run:
`bustools capture -o cDNA_capture/ -c ./cDNA_tx_to_capture.txt -e…
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Hi,
I am just starting to do some RNA Velocity analysis and it's been working so far, but I have one strange issue and I was wondering if you can see what's wrong.
I am working on the 10x Genomics…
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Hi,
I'm looking to do some RNA velocity analysis, but with data from the BD Rhapsody. It looks like for me to to use the loompy fromfq command, I'll need to build a genome index - however, in the n…
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Hi,
I am having trouble trying to follow the tutorial (http://velocyto.org/velocyto.py/tutorial/cli.html#run-dropest-run-on-dropseq-indrops-and-other-techniques).
The data I was working with were pr…
bxxu updated
5 months ago
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Hello scVelo,
We’re unable to retrieve spliced/unspliced RNA from single nuclei isolation procedure of scRNA-seq Flex kit. Please find the attached figure.
![Comparison](https://github.com/user-atta…